The purpose of this guide is to help healthcare leaders (such as chief executives and chairs), HR directors, those responsible for equality, diversity and inclusion (EDI) work, and LGBTQIA+ staff groups understand the needs of trans and non-binary colleagues, so that they can perform their duties to trans and non-binary staff and provide high-quality allyship for their workforce. It also discusses challenges that may arise as a result of being an ally, and laws to understand in relation to trans and non-binary people’s rights.